Probing RNA Structures with Hydroxyl Radicals
نویسندگان
چکیده
منابع مشابه
Massive parallel-sequencing-based hydroxyl radical probing of RNA accessibility
Hydroxyl Radical Footprinting (HRF) is a tried-and-tested method for analysis of the tertiary structure of RNA and for identification of protein footprints on RNA. The hydroxyl radical reaction breaks accessible parts of the RNA backbone, thereby allowing ribose accessibility to be determined by detection of reverse transcriptase termination sites. Current methods for HRF rely on reverse transc...
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ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2000
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc0605s00